ISHIKAWA-Luc cell line

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Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Cell Monoclonal Culture Medium

Monoclone Genotype Validation Kit

ISHIKAWA-Luc Order

Catalog#

YC-D023-Luc-P

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Datasheet

Order

Detailed Product Information

Product category

Luciferase Stable Cell Lines

cell name

ISHIKAWA-Luc

Morphology

Epithelial-like, adherent

Passage ratio

1:3-1:4

Species

Human

Reporter gene

Luciferase

Resistance gene

puro

Construction method

Lentivirus

Mycoplasma test

negative

Culture method

84%MEM+15%FBS+1%NEAA

Luciferase validation

Sample	Compound pore 1	Compound pore 2	Compound pore 3	Average	Expressed multiple
ISHIKAWA-Luc	1010650	951883	1040210	1000914.333	348.386
ISHIKAWA	2917	2861	2841	2873.0	348.386

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:ISHIKAWA				STR Info (Cell bank) Cell Line:Ishikawa
Loci	Allele1	Allele2	Allele3	Allele4	Allele1	Allele2	Allele3
D5S818	10	11			10	11
D13S317	9	12			9	12
D7S820	9	10			9	10
D16S539	9	9			9	9
VWA	14	17			14	17
TH01	9	10			9	10
AMEL	X	X			X	X
TPOX	8	8			8	8
CSF1PO	12	13			11	12

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use luciferase gene expressing lentivirus constructed by Ubigene to infect the ISHIKAWA cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a ISHIKAWA-Luc cell line that can maintain long-term, stable and high expression.

Product Advantage

Easy to use: Luciferase Stable Cell Line in our cell bank can stably express luciferase. It can be directly used for in vivo injection to detect the tumorigenicity of cells.
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Sensitive accurate：Luciferase Stable Cell Line in our cell bank have high specificity and high sensitivity, enables high imaging quality, and its luminescence intensity can be accurately quantified.
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High-quality cell: Our Luciferase Stable Cell Lines have been optimized and have low passages, high activity and good cell condition, applicable for detection or tumorigenesis experiment.
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